王江波,吳翠云,張銳

摘要：采用SSAP分子標(biāo)記技術(shù)對(duì)田間自然產(chǎn)生的紅富士蘋(píng)果（Malus domestica）新芽變材料及其對(duì)照進(jìn)行了遺傳鑒定。結(jié)果表明，一個(gè)376 bp DNA片段為芽變材料特異性片段，其序列與蘋(píng)果查爾酮合成酶（CHS）基因啟動(dòng)子同源性達(dá)90%，表明芽變材料是遺傳物質(zhì)發(fā)生改變的穩(wěn)定變異，為進(jìn)一步研究芽變材料發(fā)生性狀變異的分子機(jī)理奠定基礎(chǔ)。

關(guān)鍵詞：蘋(píng)果（Malus domestica）；芽變；SSAP；分析

中圖分類(lèi)號(hào)：Q789；S661.1 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：0439-8114（2013）05-1181-04

SSAP Analysis of Red Fuji Apple Bud Mutation

WANG Jiang-bo1a，2，WU Cui-yun1a，2，ZHANG Rui1b，2

（a.College of Plant Science；b. College of Life Science，Tarim University， Alar 843300， Xinjiang， China；2.Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin， Xinjiang Production and Construction Corps， Alar 843300， Xinjiang， China）

Abstract： Fuji apple bud mutation and the wild type were analyzed by SSAP. The results showed that a DNA fragment of 376 bp in length was specific fragment of Fuji apple bud mutation， and it was up to 90% homology with the promoter of chalcone synthase（CHS） gene. It indicated that Fuji bud mutation was stable mutation of the genetic material changes， and laid a basis for further research on the molecular mechanism of bud mutation.

Key words： apple（Malus domestica）； bud mutation； SSAP； analysis