蔡慶 黃陽全 羅樂 周皓

(重慶工程職業(yè)技術(shù)學(xué)院礦業(yè)與環(huán)境工程學(xué)院 重慶 402260)

?

厭氧氨氧化顆粒污泥的培養(yǎng)及影響因素*

蔡慶 黃陽全 羅樂 周皓

(重慶工程職業(yè)技術(shù)學(xué)院礦業(yè)與環(huán)境工程學(xué)院 重慶 402260)

厭氧氨氧化 顆粒污泥 N2H4COD Fe3+

0 引言

1 材料與方法

1.1 實(shí)驗(yàn)裝置、用水及接種污泥

1.2 實(shí)驗(yàn)方案

1.3 檢測項(xiàng)目及分析方法

2.1 反應(yīng)器運(yùn)行情況

反應(yīng)器啟動過程中污泥的粒徑分布見圖2。接種污泥體積平均粒徑為74 μm，見圖2(a)；在第31 d污泥體積平均粒徑增長到109 μm，見圖2(b)；在第93 d污泥體積平均粒徑增長到307.5 μm，見圖2(c)，此時(shí)反應(yīng)器中的污泥為絮狀和顆粒狀污泥混合物。強(qiáng)烈的水力選擇壓力對顆粒污泥的形成至關(guān)重要[15]；但水力選擇壓力又不能太高，否則會使反應(yīng)器內(nèi)ANAOB大量流失，本文通過保持合適升流速率(4.5 m/h)[8]成功培養(yǎng)得到厭氧氨氧化絮狀和顆粒狀污泥混合物。Vlaeminck等[16]采用類似的條件在SBR中培養(yǎng)出CANON顆粒和絮狀污泥的混合物，顆粒污泥占污泥VSS體積的57%，平均粒徑1.8 mm；絮狀污泥占污泥VSS體積的43%。

(a)反應(yīng)器中質(zhì)量濃度變化情況

(b)總氮去除效率及去除速率情況

(a)第1 d

(b)第31 d

(c)第93 d

2.3 N2H4對厭氧氨氧化的影響

圖4 不同質(zhì)量濃度N2H4對厭氧氨氧化的影響

2.4 COD對厭氧氨氧化的影響

圖5 不同質(zhì)量濃度COD對厭氧氨氧化的影響

2.5 Fe3+對厭氧氨氧化的影響

圖6 不同濃度的Fe3+對厭氧氨氧化的影響

3 結(jié)論

(1)在EGSB反應(yīng)器中成功啟動厭氧氨氧化過程,總氮去除速率和總氮去除效率分別達(dá)到0.931±0.006 kg/(m3·d)和90.5%±0.8%。培養(yǎng)得到厭氧氨氧化顆粒污泥和絮狀污泥混合物，污泥平均粒徑為307.5 μm。

[1]蔡慶, 張代鈞, 丁佳佳. 全自養(yǎng)脫氮顆粒污泥的培養(yǎng)及脫氮性能的恢復(fù)與強(qiáng)化[J].中國環(huán)境科學(xué),2014，34(11):2805-2812.

[2]ISAKA K，SUMINO T，TSUNEDA S. High nitrogen removal performance at moderately low temperature utilizing anaerobic ammonium oxidation reactions[J]. Journal of Bioscience and Bioengineering, 2007，103(5):486-490.

[3]STROUS M, KUENEN J G, JETTEN M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology, 1999，65(7):3248-3250.

[4]NI Shouqing, NI Jianyuan, HU Deliang ,et al. Effect of organic matter on the performance of granular anammox process[J]. Bioresource Technology, 2012，110(4):701-705.

[5]GUVEN D，DAPENA AB，SCHMID MC, et al. Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria[J]. Applied and Environmental Microbiology, 2005，71(2):1066-1071.

[6]ZEKKER I，KROON K，RIKMANN E, et al. Accelerating effect of hydroxylamine and hydrazine on nitrogen removal rate in moving bed biofilm reactor[J]. Biodegradation, 2012，23(5):739

-749.

[7]張蕾,鄭平,胡安輝. 鐵離子對厭氧氨氧化反應(yīng)器性能的影響[J].環(huán)境科學(xué)學(xué)報(bào),2009，29(8):1629-1634.

[8]ZHANG D，CAI Q，CONG L. Enhancing completely autotrophic nitrogen removal over nitrite by trace NO2addition to an AUSB reactor[J]. Journal of Chemical Technology and Biotechnology, 2010，85(2):204-208.

[9]YAO Z B，CAI Q，ZHANG D J, et al. The enhancement of completely autotrophic nitrogen removal over nitrite (CANON) by N2H4addition[J]. Bioresource Technology, 2013，146:591-596.

[10]國家環(huán)境保護(hù)總局《水和廢水監(jiān)測分析方法》編委會.水和廢水監(jiān)測分析方法[M]. 北京:中國環(huán)境科學(xué)出版社, 2002.

[11]WATT G W，CHRISP J D，CHEM A. Spectrophotometric method for determination of hydrazine[J]. Analytical Chemistry, 1952，24(12):2006-2008.

[12]GEORGE M, NAGARAJA K, BALASUBRAMANIAN N. Spectrophotometric determination of hydrazine[J]. Talanta, 2008，75(1):27-31.

[13]TANG C J，ZHENG P，WANG C H, et al. Performance of high-loaded ANAMMOX UASB reactors containing granular sludge[J]. Water Research, 2011，45(1):135-144.

[14]DAPENA-MORA A，CAMPOS J L，MOSQUERA-CORRAL A, et al. Stability of the ANAMMOX process in a gas-lift reactor and a SBR[J]. Journal of Biotechnology,2004，110(2):159-170.

[15]LIU Y，WANG Z W，QIN L, et al.Selection pressure-driven aerobic granulation in a sequencing batch reactor[J]. Applied Microbiology and Biotechnology, 2005，67(1):26-32.

[16]VLAEMINCK S E, CLOETENS L F F, CARBALLA M, et al. Granular biomass capable of partial nitritation and anammox[J]. Water Science and Technology, 2009，59(3):609-617.

[17]LOTTI T，VAN WR，KLEEREBEZEM R ,et al. The effect of nitrite inhibition on the anammox process[J]. Water Research, 2012，46(8):2559-2569.

[18]FERNNDEZ I，DOSTA J，F(xiàn)AJARDO C, et al. Short-and long-term effects of ammonium and nitrite on the Anammox process[J]. Journal of Environmental Management, 2012，95:170-174.

[19]STROUS M，PELLETIER E，MANGENOT S, et al. Deciphering the evolution and metabolism of an anammox bacterium from a community genome[J]. Nature, 2006，440(7085):790-794.

The Cultivation and the Effecting Factors of ANAMMOX Granular Sludge

CAI Qing HUANG Yangquan LUO Le ZHOU Hao

(SchoolofMiningandEnvironmentalEngineering,ChongqingVocationalInstituteofEngineeringChongqing402260)

anaerobic ammonium oxidation granular sludge N2H4COD Fe3+

重慶市教委科學(xué)技術(shù)研究項(xiàng)目(KJ1403202)。

蔡慶,男, 1985年生，湖北荊門人,博士,講師,主要從事廢水處理理論與技術(shù)方面的研究。

2015-05-28)