Guangyi Cao,Mingzhe Li,Hao Wang,Lanying Shi,You-Qiang Su,2,3,4,?

1State Key Laboratory of Reproductive Medicine,Nanjing Medical University,Nanjing,Jiangsu 211166,China;

2The Third Af filiated Hospital,Guangzhou Medical University,Guangzhou,Guangdong 510150,China;

3Collaborative Innovation Center of Genetics and Development,Fudan University,Shanghai 200433,China;

4Key Laboratory of Model Animal Research,Nanjing Medical University,Nanjing,Jiangsu 211166,China.

Supplementary Table 1 Primers for preparing template for in-vitro transcription

Supplementary Table 2 Sequences of sgRNA and donor DNA for generation of Marf1-eGFP knockin mice.The sequences of the homology arms are written in lowercase,while the eGFP coding sequence is in uppercase.

Supplementary Fig.1 DNA sequencing chromatograph showing correct fusion of the eGFP-coding sequence at the very 3′-end of Marf1 coding sequence