廖芳,陳偉偉
摘要:采用RT-PCR、Western Blot、L-[35S]放射性標記物摻入等方法,觀察甘蔗蠟提取物多廿烷醇對Hep G2細胞羥甲基戊二酸輔酶A(HMG-CoA)還原酶mRNA轉錄及蛋白質表達量的影響,及其對HMG-CoA還原酶合成和降解的作用。結果表明,多廿烷醇降低HMG-CoA還原酶mRNA的轉錄和蛋白質的表達量,且作用效果隨多廿烷醇濃度的升高而增強。此外,多廿烷醇抑制HMG-CoA還原酶的合成并加速其降解,多廿烷醇處理后HMG-CoA還原酶的半衰期明顯縮短。
關鍵詞:多廿烷醇;Hep G2細胞;羥甲基戊二酸輔酶A(HMG-CoA)還原酶
中圖分類號:R285.5 文獻標識碼:A 文章編號:0439-8114(2013)05-1125-03
Study on the Hypolipidemic Mechanism of Policosanol Extracted from Sugarcane Wax
LIAO Fang,CHEN Wei-wei
(Department of Pharmacology, Gannan Medical University, Ganzhou 341000, Jiangxi, China)
Abstract: The effects of policosanol extracted from sugarcane wax on mRNA transcription and protein expression level, synthesis and degeneration of 3-hydroxy-3-methylglutarl-coenzyme (HMG-CoA) reductase in Hep G2 cell were determined by RT-PCR, Western Blot and L-[35S] radioactive isotope tracing technology. The results showed that the mRNA transcription and protein expression level of HMG-CoA reductase decreased with the increase of policosanol concentration(10,20,30 μg/L). Policosanol also suppressed the synthesis of HMG-CoA reductases while sped up its degeneration; the half life of HMG-CoA reductases treated by policosanol was shortened obviously.
Key words: policosanol; Hep G2 cell; 3-hydroxy-3-methylglutarl-coenzyme (HMG-CoA) reductase