微生物學(xué)

封面介紹：Figure 3. Bacterial cultures and antibiotic treatment. Scale bars： A，2mm； B-F，20μm. A： the bacterial colonies cultured on LB agar for 3 days are round and opaque with neat edges. The surfaces are smooth，and shiny with pale yellow. B： the bacterium cultured with DMEM is motionless，and attached to the floor of culture vessels. C and D show almost no rod-like structures in RAW 264.7 and SH-SY5Y cultures after antibiotic treatment，respectively. However，the cell surface of SH-SY5Y exhibits numerous spine-like structures. E and F are used as controls，showing a large number of rod-like structures in the RAW 264.7 and SH-SY5Y cultures without antibiotics，respectively.

人類及小鼠某些細(xì)胞系培養(yǎng)中苯基桿菌的污染

熊天慶，王登莉，譚百宏，等

目的：對細(xì)胞培養(yǎng)體系中出現(xiàn)的桿狀污染物進行分離和鑒定，并探討如何清除該污染物.方法：采用固體培養(yǎng)基平板劃線法分離細(xì)菌株，通過熒光染色和透射電鏡對其進行形態(tài)學(xué)觀察；結(jié)合16S rRNA基因序列分析，進行菌株鑒定；用生長狀態(tài)良好的細(xì)胞上清復(fù)蘇凍存的已經(jīng)污染的細(xì)胞，檢測細(xì)胞復(fù)蘇的存活率.結(jié)果：該污染物經(jīng)形態(tài)學(xué)和16S rRNA基因序列鑒定為苯基桿菌.形態(tài)學(xué)觀察表明它有一個二態(tài)生命周期：即游動期和附著期.大多數(shù)情況下該菌可以與宿主細(xì)胞共生，常規(guī)抗生素均不能徹底清除該細(xì)菌.采用生長狀態(tài)良好的細(xì)胞上清復(fù)蘇凍存細(xì)胞可以明顯提高了細(xì)胞的存活率.結(jié)論：本實驗報導(dǎo)了苯基桿菌的二態(tài)生命周期，同時我們發(fā)現(xiàn)用細(xì)胞上清復(fù)蘇凍存細(xì)胞可以顯著提高細(xì)胞的存活率.

細(xì)胞培養(yǎng)；苯基桿菌；污染物

來源出版物：微生物學(xué)報，2015，55（2）： 176-186聯(lián)系郵箱：Yanchao Li，liyanchao@ jlu.edu.cn