Xingbing PAN，Zongjin ZHANG，Liangyu PANG，Yu GUAN，Ying WANG

1.Panzhihua Branch of Sichuan Tobacco Company，Panzhihua 617026，China；

2.Soil and Fertilizer Institute，Sichuan Academy of Agricultural Sciences，Chengdu 610000，China

Relationships between Application Amount of Quicklime and Soil Nutrients，F(xiàn)lue-cured Tobacco Quality in Panzhihua City

Xingbing PAN1，Zongjin ZHANG1，Liangyu PANG2*，Yu GUAN2，Ying WANG2

1.Panzhihua Branch of Sichuan Tobacco Company，Panzhihua 617026，China；

2.Soil and Fertilizer Institute，Sichuan Academy of Agricultural Sciences，Chengdu 610000，China

［Objective］This study aimed to investigate the improving effect of quicklime on acid soil in tobacco-growing areas in Panzhihua City.［Method］The effects of four application amounts （450，900，1 350 and 1 800 kg/hm2）of quicklime on soil nutrients and flue-cured tobacco quality were observed for two consecutive years. ［Result］In acid soil，the application of quicklime significantly improved soil pH.In addition，it showed significant effects on soil nutrients，flue-cured tobacco quality，tobacco output and tobacco leaf chemical components.When the application amount of quicklime was increased to 1 350 kg/hm2，the improving effect of quicklime on soil nutrients was best，the flue-cured tobacco yield and quality all reached the highest，and the chemical components of tobacco leaves were closest to the inherent indicators of high-quality tobacco leaves. ［Conclusion］This study will provide certain reference for accurate application of quicklime in tobacco-growing area in Panzhihua City.

Panzhihua；Quicklime；Soil nutrients；Flue-cured tobacco；Quality

P anzhihua islocated atthe junction of Sichuan and Yunnan，and at the interchange between Jinsha River and Yalong River.It belongs to Hengduan Mountains and Panxi Rift.Panzhihua has a subtropical climate with abundant rainfall，hot rainy season，suitable temperature，heavy night rain，full sunshine and appropriate air humidity，which are allfavored by flue-cured tobacco. Therefore，Panzhihua is one of the most suitable areas in the national tobacco planting regionalization.However，the soil in Panzhihua is dominated by acid yellow-red soil，and the area of soil with pH lower than 5.5 accounts for 15.87%of the total soil area［1］.Soil acidification is a widespread natural process during the formation and development of soil.Under natural rainy conditions，precipitation greatly exceeds evaporation.In addition，soil leaching is very strong，leading to downward movement of soil slat ions along with percolating water.Thus，the soluble salts in the soil are reduced. The base ions on the exchange sites of soil cations will be replaced by hydrogen ions，reducing soil base saturation degree，but increasing soil hydrogen saturation degree.Thus，soil acidification is aggravated.Soil pH is an important soil chemical property，and is also an important indicator of soil fertility［2］.Soil pH is of great significance for microbial activity，mineral effectiveness and organic matter decomposition，affecting release，fixation and migration of soil nutrients［3］.Soil pH also has great effects on yield and quality formation of flue-cured tobacco［4-7］.In recent years，there have been some studies on effects of soil pH on soil properties and flue-cured tobacco yield and quality in China［9-11］. Tobacco-growing area in Panzhihua City is one part of the high-quality tobacco-growing area in Sichuan Province［7］.There are rare comprehen-sive reports on pH of tobacco-growing soil on soil nutrients and flue-cured tobacco yield and quality.In this study，the effects of application amount of quicklime on soil nutrients and fluecured tobacco yield and quality were investigated，which has important guiding significance for soil improvement and rational fertilization in tobacco-growing area in Panzhihua City.A two-consecutive-year field test was conducted so as to comprehensively analyze the effects ofapplication amount of quicklime on soil nutrients and flue-cured tobacco yield and quality.This study will provide certain reference for accurate fertilization in tobacco-growing area in Panzhihua City.

Materials and Methods

Test time and site

The test was conducted in the Wanchang Village，Caochang Town，Miyi County，Panzhihua City，Sichuan Province during 2012-2013.The test site has an altitude of 1 688 m with annual rainfall of 1 200-1 400 mm，annual average temperature of 14-16℃and annual sunshine time of 3 000-3 400 h.

Test materials

The test materials included fluecured tobacco cultivar Honghua Dajinyuan，quicklime and yellow soil（pH 5.0）.

Test design

Total four application amounts of quicklime were designed，including 450，900，1 350 and 1 800 kg/hm2.The treatment without application of quicklime was treatment as the control. Thus，total five treatments were designed in the test.The randomized block design was adopted with three replicates for each treatment.The quicklime was buried in holes 10 d before the transplanting oftobacco seedlings.After the application，the quicklime was mixed with surrounded soil.There were about 50 tobacco seedlings in each plot.The other cultivation techniques and management were in line with the Local Standards System for High-quality Flue-cured Tobacco in Panzhihua City.

lndicator survey and analysis

Soil nutrients After the test，one soil sample was collected at the depth of 0-20 cm for each treatment.Subsequently，the soil pH and nutrients（available nitrogen，available phosphorus，available potassium，calcium and magnesium）were analyzed.

The organic matter content was determined by potassium dichromatevolumetric method［12］；the total nitrogen content was determined by Kjeldahl method［13］；the total phosphorus content was determined by spectrophotometry［14］；the pH was measured by acidometer［12］；the available nitrogen content was determined by alkaline hydrolysis diffusion method［15］；the available phosphorus contentwas determined by spectrophotometry［12］；the available potassium content was determined by flame photometry［16］；the exchangeable calcium and magnesium contents were determined by atomic absorption method；the available boron content was determined by curcumin colorimetry［12］.

Tobacco yield The tobacco leaves harvested from each plot were cured singly.In each plot，the yield，output，proportion of first-and middle-grade tobacco leaves，and average price were recorded.

Tobacco quality After the curing，the intrinsic quality（potassium content，total nitrogen content，total sugar content，reducing sugar content，nicotine content and chloride ion content）of the tobacco leaves was analyzed.

The total nitrogen content was determined by Kjeldahl method［17］；the nicotine content was determined by ultraviolet spectrophotometry［17］；the total sugarcontentand reducing sugar content were determined by Munson-Walker method［17］；the chloride ion content was determined by potentiometric titration［18］；the potassium content was determined by flame photometry［19］.

Results and Analysis

Effect of different application amount of quicklime on soil nutrients

As shown in Table 1，with the increased application amount of quicklime，soil pH and calcium content were increased gradually.The available potassium and available phosphorus contents in quicklime-applied soil were all higher than those in quicklime-free soil.When the application amount ofquicklime was increased to 150 kg/hm2，soil organic matter，available potassium， available phosphorus，available nitrogen（alkali-hydrolyzable nitrogen），total nitrogen，total phosphorus and total potassium contents were all higher，but the magnesium and available boron contents were lower than those in the control.With the increased soil alkalinity，the fixing effect of soil on magnesium was enhanced，leading to increased adsorbing effect of soil on magnesium［20-21］.In addition，the increased soil alkalinity resulted in specific adsorption of magnesium by soil［22］.

Table 1 Effect of different application amount of quicklime on soil nutrients

Effect of different application amount of quicklime on flue-cured tobacco yield

The yields and outputs of tobacco leaves in various treatment groups were shown in Table 2.The yields and outputs of tobacco leaves in quicklime-applied soilwere higherthan those in the control.When the application among of quicklime was lower than 1 350 kg/hm2，the yield and output of tobacco leaves were all increased with the increase of applica-tion amount of quicklime.However，when the application amount of quicklime exceeded 1 350 kg/hm2，the yield and output of tobacco leaves were all trended to be decreased.

Table 2 Effect of different application amount of quicklime on flue-cured tobacco yield

Effect of different application amount of quicklime on flue-cured tobacco quality

As shown in Table 3，with the increased application amount of quicklime，the variations in chemical components of tobacco leaves showed three kinds of trends.The potassium content，total nitrogen content and nicotine content in tobacco leaves were reduced with the increased application amount of quicklime.When the application amount of quicklime was lower than 1 350 kg/hm2，the total sugar and reducing sugar contents intobaccoleaveswereincreased with the increased application amount of quicklime；but when the application amount of quicklime exceeded 1 350 kg/hm2，the total sugar and reducing sugar contents in tobacco leaves were reduced with the increased application amount of quicklime.There was no significant correlation between application amount of quicklime and chloride ion content in tobacco leaves.Under normal conditions，the contents of chemical components in tobacco leaves are as follows: total sugar 18%-24%，reducing sugar 16%-22%，total nitrogen 1.5%-2.2%，nicotine 2.0%-3.5%，sugar-nicotine ratio 6-10，nitrogen-nicotine ratio 0.8-1.0，potassium ion content＞2%，chloride ion content＜0.6%［23］.In the case of non-application of quicklime，internal chemicalindicators oftobacco leaves are difficult to achieve highquality standards. However，after quicklime wasapplied in tobaccogrowing soil according to the amount of 1 350 kg/hm2，the internal chemical components of tobacco leaves were all close to the high-quality standards.

Conclusions and Discussion

Soil acidification has become an important factor affecting plant production and ecological environment［24］. The improving effect of acid soil is related to the properties of soil amendments and the properties of soil itself. At the same time，economic benefit is also needed to be taken into account.Although there are various soil amendments on market，quicklime is most commonly used in improvement of acid soil due to its wide source and cheap price.Application of quicklime has great effects on soil nutrients and flue-cured tobacco yield，quality and internal chemical components.Quicklime becomes alkaline after it is dissolved in water，and can neutralize a large number of acidic substances in soil.At the same time，quicklime can neutralize active acid and latent acid in soil.Application of quicklime is an economic and convenient method to improve acid soil.However，abuse application of quicklime may exacerbate acidification of the soil.Even worse，excessive application of quicklime may inhibit the growth of crops［24］.Therefore，rational application of quicklime is essential.

Table 3 Effect of different application amount of quicklime on flue-cured tobacco quality

With the increased application amount of quicklime，soil pH and calcium content were all increased gradually，but magnesium and available boron contents were trended to be decreased.When quicklime was applied in soil，a large number of hydroxyl ions were produced.The hydroxyl ions combined with magnesium，iron and other metal ions in soil，resulting in the generation of hydroxide precipitation. Thus，soil pH was increased，and soil acidity was neutralized.The aluminum toxicity was reduced.In addition，the application of quicklime increased exchangeable calcium content，as well as nutrients contents，in soil.When the application amount of quicklime was 1 350 kg/hm2，soil nutrients achieved a relatively optimal status for producing high-quality flue-cured tobacco.

When the application amount of quicklime ranged within 1 350 kg/hm2，the yield and output of tobacco leaves were trended to be increased with the increased application amount of quicklime.However，when the application amount of quicklime exceeded 1 350 kg/hm2，the yield and output of tobacco leaves were all decreased.It suggests that 1 350 kg/hm2is the optimum threshold application amount of quicklime for improving acid soil.In tobacco-rice rotating system （first-season crop was tobacco，and secondseason crop was rice），Tang et al.applied quicklime （0.2-0.4 g/kg）in acid soil（pH 5.0）.The results showed that the growth of local-season flue-cured tobacco was significantly improved，but the excessive application of quicklime was detrimental to tobacco growth［25］.This study found that the application amount of quicklime also could not be excessive.Frequent application of quicklime may exacerbate acidification of the soil.Even worse，excessive application of quicklime may also inhibit plant growth.Mobility of quicklime is poor in soil.Long-term and excessive application of quicklime can cause compaction of surface soil，which is not conducive to the growth ofplants，and may also lead to imbalance of soil nutrients.

When the application amount of quicklime was 1 350 kg/hm2，the internal chemical indicators of flue-cured tobacco leaves were closest to highquality flue-cured tobacco standards.

In short，application of quicklime can neutralize soil acidity，improve soil fertility and restore productivity of acidic soil，which is of great significance for sustainable development of agriculture and protection of ecological environment.In tobacco-growing area of Panzhihua City，the optimum application amount of quicklime is 1 350 kg/hm2.Under that application amount of quicklime，soil nutrients and tobacco leaf yield，quality and intrinsic quality all will be improved.

［1］GUO MQ（郭明全），HU JX（胡建新），ZHANG ZJ（張宗錦）.pH distribution and its relationship with soil available nutrient in Panzhihua tobacco lands（攀枝花煙區(qū)土壤pH分布特點及其與土壤有效養(yǎng)分的關(guān)系）［J］.Southwest China Journal of Agricultural Sciences（西南農(nóng)業(yè)學(xué)報），2012，25（4）:1338-1342.

［2］JIAKA LT（甲卡拉鐵），LI GZ（李桂珍），ZHU LX（朱俐遐），et al.Correlation between soil pH and the contents of available nutrients in selected soils from mango orchards in Panxi（攀西杧果園土壤pH值與有效養(yǎng)分的相關(guān)性研究）［J］. South China Fruits（中國南方果樹），2011，40（4）:8-12.

［3］LIU SQ（劉世全），ZHANG ZJ（張宗錦），WANG CQ （王昌全），et al.Acidity of acid soils in Tibet（西藏酸性土壤的酸度特征）［J］.Acta Pedologica Sinica（土壤學(xué)報），2005，42（2）:211-218.

［4］LIANG BJ（梁頒捷），LIN Y（林毅），ZHU QQ（朱其清），et al.Correlation between pH value and effective nutrition in tobacco soil of Fujian Province（福建植煙土壤pH值與土壤有效養(yǎng)分的相關(guān)性）［J］.Chinese Tobacco Science（中國煙草科學(xué)），2001（1）:25-27.

［5］XU ZC（許自成），WANG L（王林），XIAO HQ （肖漢乾）.pH distribution and relationship to soil nutrient in Hunan tobacco lands（湖南煙區(qū)土壤pH分布特點及其與土壤養(yǎng)分的關(guān)系）［J］.Chinese Journal of Eco-Agriculture（中國生態(tài)農(nóng)業(yè)學(xué)報），2008，16（4）:830-834.

［6］CHEN CY（陳朝陽）.The relationship of pH and available nutrient in Nanping tobacco-growing soil（南平市植煙土壤pH狀況及其與土壤有效養(yǎng)分的關(guān)系）［J］. Chinese Agricultural Science Bulletin（中國農(nóng)學(xué)通報），2011，27（5）:149-153.

［7］WANG H（王暉），XING XJ（邢小軍），XU ZC（許自成）.Relationship between pH value and available nutrients of purple soil in Panxi tobacco-growing areas（攀西煙區(qū)紫色土pH值與土壤養(yǎng)分的相關(guān)分析）［J］.Soil andFertilizer SciencesinChina（中國土壤與肥料），2007（6）:19-22，49.

［8］CHEN FR（陳發(fā)榮），CUI YH（崔永和），ZHANG LM（張立猛），et al.Data analysis of tobacco soil nutrient content in Yuxi City based on OLAP（基于OLAP的玉溪植煙土壤養(yǎng)分含量數(shù)據(jù)分析）［J］. Journal of Library and Information Sciences in Agriculture（農(nóng)業(yè)圖書情報學(xué)刊），2011，23（8）:48-51.

［9］TANG LN（唐莉娜），XIONG DZ（熊德中）. Effects of applying lime on the properties of acid soil and the leaves quality in flue-cured tobacco（酸性土壤施石灰對土壤性質(zhì)與烤煙品質(zhì)的影響）［J］.Chinese Journal of Eco-Agriculture（中國生態(tài)農(nóng)業(yè)學(xué)報），2003，11（3）:80-83.

［10］CHEN HC（陳厚才）.Quality improvement of tobacco by applying lime to acid soil（施用石灰改良酸性土壤提高烤煙產(chǎn)質(zhì)）［J］.Tobacco Science&Technology（煙草科技），1996（6）:36-37.

［11］XU Q（徐茜），ZHOU ZQ（周澤啟），WU CB（巫常標(biāo)）.Study on reducing nitrogen content and improving yield and quality of flue-cured tobacco by liming to acid soils（酸性土壤施用石灰對降低氮素及提高烤煙產(chǎn)質(zhì)的研究）［J］.Chinese Tobacco Science（中國煙草科學(xué)），2000（4）:42-45.

［12］National Agricultural Technology Extension and Service Center（全國農(nóng)業(yè)技術(shù)推廣服務(wù)中心），Institute of Agricultural Resources and Agriculture Division，Chinese Academy of Agricultural Sciences（中國農(nóng)業(yè)科學(xué)院農(nóng)業(yè)資源與農(nóng)業(yè)區(qū)劃研究所），Huazhong Agricultural University（華中農(nóng)業(yè)大學(xué)），et al. NY/T 1121.6-2006 Determination of soil organic matter content（土壤有機質(zhì)的測定）［S］.Beijing:China Agriculture Press（北京:中國農(nóng)業(yè)出版社），2006.

［13］China Agricultural University（北京農(nóng)業(yè)大學(xué)）.GB 7173-1987 Determination of soil total nitrogen content（土壤全氮測定法）［S］.Beijing:China Agriculture Press（北京:中國農(nóng)業(yè)出版社），1987.

［14］Central Laboratory of Sichuan Academy of Agricultural Sciences（四川省農(nóng)業(yè)科學(xué)院中心實驗室）.GB 9836-1988 Determination of soil total potassium content（土壤全鉀測定法）［S］.Beijing: China Agriculture Press（北京:中國農(nóng)業(yè)出版社），1988.

［15］National Agricultural Technology Extension and Service Center（全國農(nóng)業(yè)技術(shù)推廣服務(wù)中心），China Agricultural University（中國農(nóng)業(yè)大學(xué)），Hangzhou Soil and Fertilizer Testing Center（杭州土壤肥料測試中心），et al.NY/T 889-2004 Determination of exchangeable potassium and non-exchangeable potassium content in soil（土壤速效鉀和緩效鉀含量的測定）［S］.Beijing:China Agriculture Press（北京:中國農(nóng)業(yè)出版社），2004.

［16］National Agricultural Technology Extension and Service Center（全國農(nóng)業(yè)技術(shù)推廣服務(wù)中心），Nanjing Agricultural University（南京農(nóng)業(yè)大學(xué)），Anhui Provincial Soil and Fertilizer Station（安徽省土壤肥料總站），et al.NY 525-2011 Organic fertilizer（有機肥料）［S］. Beijing:China Agriculture Press（北京:中國農(nóng)業(yè)出版社），2011.

［17］National Tobacco Quality Supervision and Inspection Center（國家煙草質(zhì)量監(jiān)督檢驗中心）.YC/T 32-1996 Determination of water-soluble sugar（水溶性糖的測定方法）［S］.Beijing:China Standard Press（北京:中國標(biāo)準(zhǔn)出版社），1996.

［18］Qingzhou Tobacco Research Institute of China National Tobacco Corporation（中國煙草總公司青州煙草研究所）. YC/T 153-2001 Determination chloride content in tobacco and tobacco products（煙草及煙草制品氯含量的測定）［S］.Beijing:China Standard Press（北京:中國標(biāo)準(zhǔn)出版社），2001.

［19］Qingzhou Tobacco Research Institute of China National Tobacco Corporation（中國煙草總公司青州煙草研究所）. YC/T 173-2003 Determination of potassium content in tobacco and tobacco products（煙草及煙草制品鉀的測 定 ）［S］.Beijing:China Standard Press（北京:中國標(biāo)準(zhǔn)出版社），2003.

［20］YU TR （于天仁）.Electrochemistry of soil with variable charge（可變電荷土壤的電化學(xué)）［M］.Beijing:Science Press（北京:科學(xué)出版社），1996.

［21］CHUBIN RG，STEET JJ.Adsorption of cadmium on soil constituents in the presence of complexing ligands［J］. Environ Qual，1981，10（2）:225-228.

［22］LI XH（李學(xué)恒）.Soil chemistry and experimental guidance（土壤化學(xué)及實驗指導(dǎo)）［M］.Beijing:China Agriculture Press（北京:中國農(nóng)業(yè)出版社），1997.

［23］LIU GS（劉國順）.Tobacco cultivation（煙草栽培學(xué)）［M］.Beijing:Agriculture Press（北京:農(nóng)業(yè)出版社），2003.

［24］WANG N（王寧），LI JY（李九玉），XU RK（徐仁扣）.Acidification of soil and improvement and management of acid soil（土壤酸化及酸性土壤的改良和管理）［J］.Anhui Agricultural Science Bulletin（安徽農(nóng)學(xué)通報），2007，23（13）:48-51.

［25］TANG LN（唐莉娜），XIONG DZ（熊德忠），ZHANG YC（張永成），et al.Residual effect of lime application on farming system of tobacco-rice in acid soil（酸性土壤施石灰對煙稻輪作后效的影響）［J］.Journal of Fujian Agriculture and Forestry University（Natural Sciences）（福建農(nóng)林大學(xué)學(xué)報:自然科學(xué)版），2002，31（4）:517-520.

Responsible editor：Tingting XU

Responsible proofreader：Xiaoyan WU

攀枝花煙區(qū)生石灰施用量與土壤養(yǎng)分及烤煙質(zhì)量的關(guān)系

潘興兵1，張宗錦1，龐良玉2*，官宇2，汪瑩2
（1.四川省煙草公司攀枝花市公司，四川攀枝花617026；2.四川省農(nóng)業(yè)科學(xué)院土壤肥料研究所，四川成都610000）

［目的］檢測生石灰對攀枝花煙區(qū)酸性土壤的改良效果。［方法］2012～2013連續(xù)2年通過田間試驗研究4種生石灰施用量（450、900、1 350、1 800 kg/hm2）對土壤養(yǎng)分及煙葉生產(chǎn)的影響。［結(jié)果］在酸性土壤上施用生石灰，不僅對提高土壤pH有明顯作用，而且對植煙土壤養(yǎng)分、烤煙產(chǎn)量、產(chǎn)值以及煙葉內(nèi)在化學(xué)成分的變化有明顯影響。其中，當(dāng)生石灰施用量達到1 350 kg/hm2的標(biāo)準(zhǔn)時，土壤養(yǎng)分調(diào)節(jié)效果最顯著，烤煙產(chǎn)量、質(zhì)量均達到最高，煙葉內(nèi)在化學(xué)成分最接近優(yōu)質(zhì)煙化學(xué)內(nèi)在指標(biāo)。［結(jié)論］該試驗為攀枝花煙區(qū)的精準(zhǔn)化施肥提供了參考。

攀枝花；生石灰；土壤養(yǎng)分；烤煙；質(zhì)量

四川省煙草公司攀枝花市公司科技基金（攀煙司［2011］31號）；中國煙草總公司四川省公司2013年重點科技項目“烤煙中、微量元素營養(yǎng)失調(diào)癥診斷及產(chǎn)品開發(fā)”（川煙科［2013］4號）。

潘興兵（1985-），男，重慶人，助理農(nóng)藝師，從事烤煙土壤肥料與栽培研究。*通訊作者，研究員，從事耕作栽培與農(nóng)業(yè)生態(tài)研究，E-mail：272659962@qq.com。

2015-08-24

Supported by Science and Technology Fund of Panzhihua Branch of Sichuan Tobacco Company（PYS［2011］31）；Science and Technology Key Project of Sichuan Branch of China National Tobacco Company（CYK［2013］4）.

*Corresponding author.E-mail:272659962@qq.com

Received:August 24，2015 Accepted:November 17，2015

修回日期 2015-11-17