INTRO D UCTION

Diabetic retinopathy (DR) is the most common retinal vascular complication of diabetes mellitus (DM)and the leading cause of vision impairment and blindness among working-age adults. In Chinese adults, the overall standardized prevalence of diabetes using the WHO criteria is 11.2%, of which prediabetes accounts for 35.2%. DR is largely asymptomatic in the early stages, but neural retinal damage and clinically invisible microvascular changes progress during these early stages. It has therefore been widely accepted that periodic eye examinations should be conducted on all patients with DM to detect significant retinopathy andprovide prompt interventions when necessary, which is thought to be the most effective method to reduce potential DR-related visual disabilities.

孔徑分布和截留相對分子量采用一系列的PEG截留率結果并通過Matlab軟件采用下述對數(shù)正態(tài)分布方程模擬計算得到[15]：

Traditional tabletop-based, mydriatic retinal imaging modality is effective but is less available in underdeveloped rural areas. Handheld cameras are now emerging as a new low cost tool for DR screening, which can be conveniently used for patients who may not have access to ophthalmological care,with the potential of improving DR screening.

In this study, we described a new portable Optomed Aurora fundus camera, and compared the image quality and DR detection with traditional tabletop fundus cameras. We also evaluated the feasibility of using a deep learning system(Phoebus, Shanghai, China) to detect different signs of DR to determine the possibility of combining the handheld fundus camera and AI technology during DR screening.

However, it is difficult for physicians to perform routine dilated examinations on screened patients to detect non-symptomatic conditions owing to a presumption of patient unwillingness,lack of time, and unwarranted fears of harming patients with known glaucoma. Therefore, the quality of photography under non-mydriatic conditions is a critical evaluation index in DR screening. Compared to conventional cameras, the Aurora has a smaller minimum pupil size requirement (Table 2).For images taken during a non-mydriasis state, the Aurora handheld fundus camera had significantly better quality in centration, clarity, and visible range (1.47±0.46, 1.48±0.40, and 1.40±0.47, respectively) than conventional tabletop cameras(1.30±0.58, 1.28±0.19, and 1.18±0.61, respectively;＜0.001).During the mydriasis state, the Optomed Aurora color images had imaging and grading characteristics similar to those of conventional tabletop cameras. Importantly, the advantage of not requiring pupillary dilation could provide the impetus for DR screening in underdeveloped regions.

SUBJECTS AND METHODS

The study was approved by the Shanghai General Hospital Institutional Review Board at Shanghai Jiao Tong University. Informed consent was obtained from all participants. The study was conducted in compliance with the Declaration of Helsinki, in accordance with the ICH-GCP(International Conference on Harmonization-Good Clinical Practice) guidelines (clinicaltrials.gov Registration Number:NCT03903042).

Three parameters were assessed by the graders to investigate various aspects of image quality (Figure 1). For each parameter the quality score ranged from 2 (excellent) to 0 (ungradable). Images were considered to be excellent quality if the macula or optic disc was well-centered, showing clarity of the fundus vessels,with any retinopathy and the whole image being visible. If the macula or optic disc was partially centered and the fundus vessels and any retinopathy were recognizable and more than 80% of the image was visible, the images were defined as acceptable. If images were not centered, blurred without recognition of the retinal vessels or retinopathy features, or less than 80% of the image was visible, they were defined as ungradable.

All photographs were randomized and presented to three masked and experienced ophthalmologists.The ophthalmologists separately evaluated their image quality and made diagnoses without information about the patients or the cameras used.

This was a multi-centered, double-blinded,observational clinical study enrolling patients from three hospitals (Shanghai General Hospital, Shanghai, China; West Nanjing Road Community Health Center, Shanghai, China;and Zhaoqing Gaoyao People's Hospital, Guangdong, China).Individuals of ages 18y and older who had been diagnosed with DM were enrolled. Patients were excluded if the retina specialist could not visualize the fundus on examination or if they had previously undergone vitreoretinal surgery and/or laser photocoagulation. Classification of the severity stage of DR was determined using the International Clinical Diabetic Retinopathy Disease Severity Scale grading system developed by the American Academy of Ophthalmology (AAO)(Table 1).Four fundus photographs per eye were taken by a well-trained ophthalmic photographer: papilla- and maculacentered images using the tabletop and handheld Optomed Aurora fundus cameras (Optomed, Oulu, Finland), respectively,with or without pupil dilation with 1% tropicamide. The images obtained with the handheld fundus camera had a field of view of 50° and 5 mega-pixel resolution. Images from both kinds of cameras were acquired on the same day, which allowed for direct pathological identification and comparisons between these camera types. The characteristics of five types of fundus cameras are detailed in Table 2. The images were stored as JPEG (Joint Photographic Experts Group) files after removing patient names. Fundus images were transferred to the grading center, Shanghai General Hospital through the INSIGHT real-world patient registry platform www.chinadr.org.cn (Phoebus Medical, Shanghai, China), for remote digital retinal imaging grading.In this study, the images were uploaded to the deep learning system, Phoebus (Phoebus), and the detection of DR features was assessed. Phoebus provides a DR grade per image as well as visual representations of detected microaneurysms, retinal hemorrhages, hard exudations, and macular edemas. The DR output from Phoebus was further used to generate a prediction for the referral requirement.

Overall, a total of 630 eyes of 315 DM patients were included in this study. The patients were on average 65.5±11.1y of age, and 53.7% were female (=169).The median duration of diabetes was 10y (3-15y), with more than one-third of the patients diagnosed with diabetes for less than 5y, and only 20 (6.3%) patients were diagnosed with diabetes for over 20y (Table 3).

RESULTS

Statistical analysis was performed using SPSS statistical software for Windows, version 20.0 (SPSS,Chicago, IL, USA). The data are presented as the mean±SD or median (IQR) for continuous variables and frequency (%)for categorical variables. Participant age was categorized by intervals of 10y, and age at diagnosis of diabetes was categorized by intervals of 5y. Sensitivity, specificity, and area under the receiver operator curve (AUC) with a 95% confidence interval(95%CI) were calculated to evaluate diagnostic accuracy.We used a receiver operating characteristic (ROC) curve to evaluate the classification ability of our built model.

Of the 630 eyes examined, 242 eyes (38.4%)were photographed in the non-dilated state, while the remaining 388 eyes were dilated. The mean scores of the nonmydriasis image quality regarding image centration, sharpness,and visible range for the handheld fundus camera were 1.47,1.48, and 1.40, separately, resulting in a significant advantage over the conventional tabletop cameras (1.30, 1.28, 1.18,separately,＜0.001). However, regarding the image sharpness score under mydriasis, photography with the conventional tabletop cameras performed better than the images acquired by the Aurora handheld fundus camera (＜0.05). The assessment of mydriasis images regarding image centration (=0.146) and visible range (=0.945) did not reach a significant difference(Table 4).

We compared the ability to reveal common manifestations of DR between the handheld and tabletop fundus cameras (Table 5). Thesensitivity and specificity to detect microaneurysms reached 94.4% (95%CI: 87.0%-100.0%) and 98.4% (95%CI: 97.3%-99.5%), respectively, using the Aurora camera, compared to 89.7% (95%CI: 80.2%-99.3%) and 98.6% (95%CI: 97.6%-99.6%) using the conventional tabletop camera (Table 6). Detection of retinal hemorrhage, hard exudation, and macular edema were comparable, with that of the AUC of the handheld fundus camera being slightly lower. When detecting referable DR, the Aurora camera obtained an AUC of 88.2%(95%CI: 83.5%-92.8%), corresponding to a sensitivity of 82.1% (95%CI: 72.1%-92.2%) and a specificity of 97.4%(95%CI: 95.4%-99.5%), when compared to 92.7% (95%CI:85.9%-99.6%) and 95.9% (95%CI: 93.2%-98.5%) using the conventional tabletop camera. The corresponding ROC curves are shown in Figure 2.

The performance of AI detection of DR using the Phoebus Algorithm is shown in Table 6. On images taken from the handheld camera, the Phoebus Algorithm achieved an AUC of 77.8% (95%CI 70.2%-85.4%) when detecting retinal hemorrhage, corresponding to a sensitivity of 73.9% (95%CI 61.2%-86.6%) and a specificity of 81.5% (95%CI 78.0%-84.9%). When detecting hard exudation, the Phoebus Algorithm obtained an AUC of 76.7% (95%CI 70.5%-83.0%),corresponding to a sensitivity of 87.5% (95%CI 78.1%-96.9%) and a specificity of 65.4% (95%CI 61.2%-69.7%). For the referral requirements, the Phoebus Algorithm achieved an AUC of 64.8 (95%CI 59.6%-70.1%), corresponding to a sensitivity of 88.2% (95%CI 79.4%-97.1%) and a specificity of 40.7% (95%CI 34.1%-47.2%). The corresponding ROC curves are shown in Figure 3.

這自然也包括占星術，“在俘囚期時代，即使是在巴比倫當?shù)?，第二以賽亞便不止嘲諷一般的巴比倫巫師，而且特別也嘲諷了巴比倫的天文學與占星術。到了俘囚期之后與拉比時代，星宿在以色列毫無用武之地的原則依然存在。”[注][德]韋伯：《古猶太教》，康樂、簡美惠譯，桂林：廣西師范大學出版社，2007年，第268頁。 而占星術在中國是表明王朝合理性的永恒證據(jù)，儒家或儒教根本不會去觸動這一自新石器晚期巫酋時代就業(yè)已形成的根深蒂固的觀念，并且作為歷代王朝的主要輔助者或捍衛(wèi)者，儒家只會去不斷改善和強化這一傳統(tǒng)；故占星術同樣也是儒家或儒教的深厚傳統(tǒng)。

DISCUSSION

In this study, we introduced a new handheld Optomed Aurora fundus camera, and found it significantly better than traditional tabletop fundus cameras regarding image quality during the non-mydriasis state. The detections of different signs of DR were comparable with the tabletop cameras. The Aurora fundus camera combined with an autonomous AI system had high sensitivity and specificity for DR detection. Furthermore,it was safe and effective in the detection of referable DR in real practice.

During the last two decades, automated image diagnosis based on AI has been used for detection and classification of DR with the advantages of increased efficiency, reproducibility, and coverage of screening programs. However, application in real world situations remains a challenge due to inconsistent image quality and other aspects such as comorbidity. The accuracy of screening by fundus photography is highly dependent on the performance in detecting different manifestations of DR, using either manual grading or AI-assisted grading. The performances in DR screening between the Aurora and tabletop cameras were comparable in principle. It is worth noting that the Aurora was better than the tabletop cameras in screening sensitivity for detecting microaneurysms (94.4%89.7%),possibly because of the better image quality of the Aurora during the non-mydriasis state. The sensitivity and specificity of the Aurora in the detection of referral-warranted DR were 82.1% and 97.4%, respectively, which met the criteria of The British Diabetic Association, when considering 80% sensitivity and 95% specificity for a viable DR screening program.Overall, these results indicated a satisfactory quality of Aurora for AI-assisted grading.

二是參考標桿管理，對標對表明確?？瓢l(fā)展方向。結合建設西南一流、國內有較大影響力的現(xiàn)代化大型三甲綜合醫(yī)院發(fā)展目標，醫(yī)院要求各?？迫鎸恕秶覅^(qū)域醫(yī)療中心設置標準（綜合醫(yī)院）》，深入分析存在的差距與不足，通過“走出去、請進來”的方式，不斷加大外出進修學習、外請專家來院指導力度，以實現(xiàn)在日常工作中持續(xù)整改、不斷提升?？萍夹g水平的最終目的。

基于此，提出了基于生成對抗文本的人臉圖像翻譯方法，相比其他翻譯方法，本文的翻譯結果更好，在人臉圖像上具有很好的適應性。

Fundus photography has served as a useful tool in detecting and documenting the presence and the progression of retinopathy in diabetic patients in communities. On this basis, the development of digital fundus cameras further facilitates rapid acquisition and interpretation of fundus images and the rapid deployment of retinal imaging for DR screening worldwide. These cameras produce high quality images that can be assessed for the presence of DR by eye care providers (optometrists or ophthalmologists)or trained readers in a deferred manner on site or remotely.Moreover, many computer-aided algorithms for automated image analysis have been developed, which are expected to be a promising alternative for retinal fundus image analysis for future applications in eye care. Artificial intelligence(AI) systems have been widely demonstrated to lower cost,improve diagnostic accuracy, and increase patient access to DR screening. In April 2018, the United States Food and Drug Administration approved the world's first AI medical device for detecting DR, the IDx-DR.

Early detection and prompt treatment of DR is the key to reducing preventable vision loss worldwide, which requires regular fundus screening. However, because of the paucity of ophthalmologists in China, there are only about 20 practitioners per million people, which reduces the accessibility of DR screenings. Even in the United States and the United Kingdom, the absolute number of ophthalmologists (49 and 59 ophthalmologists per million people, respectively) still cannot meet the need of a growing number of DR patients,especially in rural areas. Fundus photography has been widely proven to be an effective method to monitor the extent of DR and to identify patients who could benefit from early treatment. However, the large size, weight, and high cost of the conventional tabletop fundus cameras limit its use for large-scale screening in communities lacking a sufficient screening process. In rural and remote communities with few ophthalmologists or table-top fundus cameras, teleophthalmology based on portable fundus cameras used by well-trained physicians is a viable solution to increase DR screening. Therefore, non-mydriatic portable ocular fundus photography is a promising solution when combined with telemedicine. The dimension of our new handheld Aurora fundus camera is approximately 122×202×98 mm, which is much smaller than all tabletop cameras (Table 2), making it possible to be carried and used for training for those without experience.

We used our self-designed Phoebus algorithm system to examine the performance in detecting DR. The system had independent, validated detectors for lesions characteristic of DR, including retinal hemorrhages, hard exudates, and macular edemas, the outputs of which were then fused into a DR referral requirement output, using a separately trained and validated machine learning algorithm. Several studies have reported the diagnostic performance of AI-based software in the detection of referable DR. The published results appeared promising, showing a sensitivity ranging from 74% to 92.5%,and a specificity between 73.3% and 98.5%. In the present study, the sensitivity and specificity of the Phoebus Algorithm system in identifying referable DR was 88.2% and 40.7%,respectively, which met the FDA superiority sensitivity cut-offs of 85%, but did not reach a specificity of 82.5%. However,in contrast, its sensitivity and specificity of macular edema were 44.1% and 90.1%, respectively. One of the possible reasons was that macular edema is not easily captured using 2-dimensional fundus photographs. In contrast, optical coherence tomography, as a standard diagnostic tool for the assessment of intra- and subretinal fluids, is more sensitive in detecting macular edemas. Sensitivity is a patient safety criterion, because the AI system's primary goal is to identify as many potential patients with DR that require further evaluation by eye care providers. Tanreported an algorithm that achieved a sensitivity and specificity of 62.57% and 98.93%,respectively, for retinal hemorrhages. Our detection sensitivity and specificity of retinal hemorrhages were 73.9% and 81.5%,respectively, with the corresponding AUC of 77.8%. The AIassisted grading system of both mydriatic and non-mydriatic images could therefore be valuable in DR screening.

This study had some limitations. First, the inclusion of multiples images was from multiple devices with different fields of view and resolutions. However, it could also be a strength for the resulting algorithms, which could be more reliable in the real world with different camera brands or types.Second, the AI-based specificity of referable DR was relatively low, which could affect the number of people who received a referral but did not actually need one because they had only no DR or mild DR. This could have been due to the small sample size of the study. Real-world data of more DR patients will be needed to improve the analysis.

In conclusion, our study showed that the handheld Aurora fundus camera was well-suited for DR screening with or without mydriasis. This camera had high sensitivity of DR detection as well as satisfactory image quality, but its specificity needs to be improved with better modeling of the data. Our Phoebus AI system helped to improve DR screening. Use of a handheld fundus camera with an AI system was safe and effective in the detection of referable DR in the real world practice.

葉曉曉不慌不忙，眨著單眼皮的小眼睛微微一笑，說：“從我的眼神。我的眼睛告訴你的是純真，不是隱晦和暗示什么?！?/p>

制造任務特征主要描述了該任務中區(qū)別于其他任務的典型特征信息，包括加工特征、設備特征和工藝特征等。例如某軸承加工任務，其制造任務特征為車削加工、車床、材料、尺寸等。該特征的確定一般根據(jù)制造任務所加工的產品對象的工藝過程特點來確定該制造任務執(zhí)行過程中的制造任務特征。

Supported by the National Natural Science Foundation of China (No.81970845); European Union's Horizon 2020 research and innovation programme under grant agreement (No.778089).

None;None;is an employee of Shanghai Phoebus Medical Co. Ltd.None;None;None;None;None;None;None.