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Neural functions of small heat shock proteins

2022-08-15 15:46:14TeresadelosReyesSergioCasasTint
中國神經再生研究(英文版) 2022年3期

Teresa de los Reyes, Sergio Casas-Tintó

Abstract Stress response is a cellular widespread mechanism encoded by a common protein program composed by multiple cellular factors that converge in a defense reaction to protect the cell against damage.Among many mechanisms described, heat shock proteins were proposed as universally conserved protective factors in the stress core proteome,coping with different stress stimuli through its canonical role in protein homeostasis.However, emerging evidences reveal non-canonical roles of heat shock proteins relevant for physiological and pathological conditions.Here, we review the implications of inducible heat shock proteins in the central nervous system physiology.In particular, we discuss the relevance of heat shock proteins in the maintenance of synapses, as a balanced protective mechanism in central nervous system development, pathological conditions and aging.

Key Words: aging; central nervous system; chaperones; environment; neuroprotection;pathology; stress; synapses

Stress Response and Chaperones

The stress response arises from common cellular stressors that alter the environment and impact on macromolecules such as proteins or DNA.In consequence, key aspects of the cell physiology are altered including oxidative stress production,redox state, cell cycle control, macromolecular stabilization and metabolism.As a result, if stress overcomes the limits of this response, the cell activates an apoptotic death program(Kültz, 2005).Moreover, gene expression changes contribute to maintain cellular dynamics (Gasch et al., 2000; Tukaj, 2020),but different stress conditions or stressors trigger diverse gene expression changes (Gasch et al., 2000; Girardot et al.,2004).Yeast stress response is a good example of this diversity in cellular adaptive mechanisms.Heat is the classical factor in stress response that promotes the expression of cytosolic,mitochondrial and endoplasmic reticulum chaperones genes,and induces the expression of genes involved in metabolism regulation.However, yeast exposure to H2О2increases reactive oxygen species production and elicits a genetic program based on cellular detoxification, redox reactions genes and heat shock genes.In addition, starvation, exposure to redox agents(dithiothreitol and diamide) or osmotic variable conditions provokes dramatic gene expression changes, making the stress response unique for each stressor (Gasch et al.,2000).This diversity shapes a suitable cellular response that facilitates the adaptation to survive in a changing environment through different species.In multicellular model organisms asDrosophila melanogasterorArabidopsis thaliana, the evidences of different gene expression profile upon different stressors support the conservation of these mechanisms in evolution (Girardot et al., 2004).

Even though each stress response is proposed to elicit a specific response, the genetic program induced by these aggressions share a common and highly conserved profile at the cellular stress proteome: the induction or activation of stress proteins including the heat shock protein (HSPs)family or molecular chaperones, DNA damage sensing/repair proteins and a set of degradation proteins (Kültz, 2005).InSaccharomyces cerevisiae, this common transcriptional profile to different stressors is known as stress core, part of the global common environmental stress response that facilitates the adaptive process (Gasch et al., 2000).Drosophila melanogasteralso shows a common transcriptional profile to different environmental conditions that includes conserved genes in the common long-term stress response (Girardot et al., 2004).Heat, salinity, hypoxia, heavy metals, radiation,pesticides or even physical activity are considered stressors due to their ability to induce stress proteins production(S?rensen et al., 2003).

Search Strategy and Selection Criteria

The articles cited in this review were collected from PubMed database after a deep research in articles published from 1999 to 2020 with the following terms: “stress response” “heat shock proteins” “molecular chaperones”, the combination of “heat shock proteins” and “central nervous system”or “neurodegeneration”, the combination of “heat shock proteins” and “synapses”.

Role of Heat Shock Proteins in the Central Nervous System

HSPs proteins were first described as stress molecules that bind to destabilized proteins and assure a correct protein folding or facilitate the degradation of misfolded proteins,maintaining cellular proteostasis network (Hipp et al., 2019)(Figure 1).Thus, HSPs are a canonical cellular protection factor as stress-inducible proteins.Nevertheless, there are constitutive HSPs that are expressed ubiquitously; these constitutive HSPs have specific functions in cell physiology.In addition to protein folding, these proteins modulate cytoskeleton protection, anti-inflammatory response,autophagy, anti-apoptotic signals and developmental functions (Bakthisaran et al., 2015).Emerging evidences reveal specific activity of cellular chaperones in neural physiology,HSPs participate in synaptic maintenance, neuronal activity and protection against neurodegenerative insults under pathological conditions.

Figure 1|Neural functions of chaperones.

HSPs in synaptic maintenance

Karunanithi et al.demonstrated that the exposure to heat stress inducesHsp70expression inDrosophila melanogaster,and correlates with synaptic transmission maintenance in the neuromuscular junction (Karunanithi et al., 1999).This mechanism is conserved in mammals: hyperthermia inducessHsp27andsHsp32expression in Bergmann glial cells bodies.Their transport via radial glial fibers to the rat cerebellum (Bechtold and Brown, 2000).allows them to take part in neurotransmission regulation and neuronal protection(Bechtold and Brown, 2000).Moreover, HSP70 provokes a neuroprotective effect and increases neuronal survival under heat shock, oxidative stress, and ischemia-like conditionsin vitroandin vivomodels (Yenari et al., 1999; Kim et al.,2020).It has been proposed that glial cells can transfer HSP70 protein to neurons and promotes neuronal stress tolerance as a neuroprotective mechanism (Guzhova et al., 2001).Given the contribution of HSPs in neuronal survival and synapse maintenance, there is a need to understand the mechanisms underlying chaperone activity modulation in the nervous system.

HSPsare expressed in neurons and glial cells, and are involved in protein folding, vesicle trafficking and neurotransmitter release (Stetler et al., 2010).In this context, molecular chaperones mediate the turnover of receptors through uncoated vesicles in postsynaptic structures, and regulate vesicle fusion and neurotransmission in presynaptic structures (Stetler et al., 2010).Small HSPs (sHSPs) are the most extended HSPs subfamily, we have shown recently inDrosophila melanogasterthat the modulation of sHSPs in neurons compromises synapse number.We described that the upregulation ofsHsp23andsHsp26in neurons provokes an increase in synapse number and neuronal function, acting as pro-synaptogenic complex during development.Moreover,the combined downregulation ofsHsp23andsHsp26does not alter synapse number.Genetic modifications that affect the expression ofsHsp23orsHsp26, provokes an imbalance in chaperones equilibrium that causes a reduction in synapse number, reinforcing the idea of chaperone complex and protein quality system equilibrium.Thus, sHSPs in neurons participate in synapse maintenance and modulation of neuronal function (Santana et al., 2020).

sHSPs balance in synapse maintenance

The chaperone quality system is critical for protein folding or degradation, in consequence it is under a fine-tuned regulation.A balance is established between folding or degradation chaperone activity and a failure in this system provokes pathological conditions (Muller et al., 2013).Different regulatory mechanisms have been proposed to modulate chaperone activity as the presence of unfolded or partially folded substrates, changes in temperature,posttranslational modifications and hetero-oligomers formation (Haslbeck and Vierling, 2015).Among these mechanisms of regulation, kinase proteins play a central role through posttranslational modifications that regulate HSPs dimer/oligomer formation balance and hence, HSPs activity(Muller et al., 2013; Haslbeck and Vierling, 2015) (Figure 2).

Figure 2|Diagram of small chaperones sHSP23, sHSP26 and Pkm mechanisms of function.

HSPs proteins typically contain phosphorylation sites susceptible to kinase proteins modifications (Muller et al.,2013; Katsogiannou et al., 2014).Un-phosphorylated sHSP27 can stablish multimers that act as molecular chaperones,besides phosphorylated sHSP27 leads to a significant decrease of oligomer size and promote complex dissociation (Rogalla et al., 1999).Moreover,sHSP27expression correlates with tumour cells resistance to chemotherapy and tumor progression (Matsushima-Nishiwaki et al., 2008; Kuramitsu et al., 2012).These results strongly suggest that the control of the phosphorylated HSP27 levels could be a new therapeutic strategy specially to counter cancer recurrence.In this context,Muller et al.(2013) modifications in the phosphorylation sites of the C-terminal region of HSP70 and HSP90.These alterations are critical for HSPs phosphorylation, activity and for the folding/degradation dynamic equilibrium that control the proliferation rate of cancer cells (Muller et al., 2013).Thus, the phosphorylation state can alter protein activity and interactions with chaperones clients as a key-mechanism to sHSPs regulation.In our recent work, we propose that that sHSP23 and 26 neuronal functions are mediated by a novel putative kinase protein CG1561, named Pinkman (Pkm)(Santana et al., 2020).

We decided to study the common interactome of sHSP23 and 26 to explore for potential modulators of their function as a complex.Both proteins share three common interactors predicted as protein kinase activity molecules: CG11534,CG43755 and CG1561 (Santana et al., 2020).Pkm is physically interacts with sHSP23 and sHSP26 (Guruharsha et al., 2011),and the absence ofpkmdoes not alter sHSP23-26 complex formation (Santana et al., 2020).However,pkmknockdown provokes an increase insHsp23expression.Pkm is involved in the transcriptional regulation ofsHSP23that, in consequence,alters sHSPs complex stability, synapse number and neuronal activity.We hypothesized that Pkm-sHSPs balance is relevant as central regulatory mechanism for the correct formation,function and response of the nervous system to environmental conditions.

sHSPs in pathology

Internal environmental stressors such as pathophysiological stress, nerve injury, axon damage or ischemia, modulateHSPsexpression in the nervous system.HSPs promote neuronal survival, axon regeneration, myelination and functional locomotor recovery (Оusman et al., 2017).In particular, HSP70 and HSP27 chaperones are well studied in nervous system damage, and have emerged as promising therapeutic target(Оusman et al., 2017).Further, increasing evidences associate chaperones strategies with a neuroprotective mechanism in neurodegeneration events (Stetler et al., 2010; Bakthisaran et al., 2015).

Nervous system protection by sHSPs

In addition to the previously described stressors, proteostasis network is vulnerable to collapse in neurons under certain conditions as aging or neurodegeneration, what is known as chaperone-overload model.In this context, an imbalance of cellular proteostasis is a hallmark in aging that induces protein aggregation, cellular dysfunction and the onset of neurodegenerative processes.HSPs system plays a central role in proteostasis network function, and participates as a defense barrier against disruptions in protein dynamics.Besides, recent publications propose HSPs function and protein levels as a brain quality system that declines with aging and neurodegeneration (Hipp et al., 2019).Within the HSPs family, sHSPs are upregulated in aged tissues in different species, and are proposed as a conserved antiaging mechanism (Hipp et al., 2019).Оur recent results and others show that overexpression of sHSPs increases synapse number (Santana et al., 2020), co-localize with aggregates in neurodegenerative-aggregated diseases and contribute to restore neural function (Stetler et al., 2010).Moreover,mutations that disrupt sHSPs structure and function are responsible of neuropathies and myopathies, known as chaperonopathies (Bakthisaran et al., 2015).For example,mutations in sHSP27 are commonly inherited as autosomal dominant mutations related to Charcot–Marie–Tooth disease(Bakthisaran et al., 2015).Besides, mutations in HSPB4 are related to retina degeneration and cataract disease (Frankfater et al., 2020).Moreover, mutations in sHSP20 sequence that affect protein phosphorylation state, affect smooth muscle contraction in cardiomiocytes (Gardner et al, 2019).

Trans Generational Regulation of Chaperones

Stressors change environmental conditions that induce the modulation of genetic programs, including changes inHSPsexpression.These changes in the DNA code facilitate the adaptation to the new conditions and therefore, the heritable information can affect the features of the next generation(Maggert, 2019).In this context, a revolutionary field is emerging based on the impact of stress in the organism, and the inheritance of genetic and epigenetic modifications across generations that compromise fitness and evolution (Heard and Martienssen, 2014; Cappucci et al., 2019; McGreevy et al., 2019).Artemiais a genus of crustacean that after heat exposure increases HSP70 levels, this upregulation improves environmental stress resistance, and it is observed in three subsequent generations, (Norouzitallab et al., 2014).Moreover, in Drosophila ovaries and testis, Hsp70 chaperone interacts with the chaperone complex and factors involved in piRNAs biogenesis after heat shock, and induces displacement of these factors to the lysosomes (Cappucci et al., 2019).Besides, stress exposure inCaenorhabditis elegansalters the expression ofHeat Shock Factor 1(HSF-1) in the germline,this transcription factor modulates epigenetics mechanisms and induces inheritable marks that improve stress resistance in the progeny (Kishimoto et al., 2017).All these evidences indicate that HSPs cope with environmental conditions, and inheritable factors that modulate gene expression condition the adaptation of the next generations to stress.This form of adaptation claims for inheritable acquired features in evolution, consistent with other studies that link stress inheritance and CNS modulation (McGreevy et al., 2019).

Conclusions

The stress response has been a hot field of study during last decades linked to the challenge of better understanding role of HSPs in cell physiology.Proteotasis network arises as a key factor in the central nervous system and includes the noncanonical roles described here.We have recently described a balanced system between sHSP23 and sHSP26 that act under the modulation of Pinkman kinase-like protein in the synapse formation and modulation (Santana et al., 2020).This implication is consistent with other studies that propose HSPs as therapeutic molecules to neuronal disturbance(Stetler et al., 2010; Bakthisaran et al., 2015; Оusman et al.,2017; Hipp et al., 2019).Here we discuss a perspective for HSPs as a sensor to the environmental conditions that targets neural function and synaptogenesis in development, aging or neurodegenerative stages.Synapse disruption is associated with morphological impairments but also with cognitive and psychiatric disorders (Das et al., 2019).Thus, HSPs emerge as a new central mechanism to assist synapse formation and maintenance, but also a potential target to drive therapeutic approaches to CNS malignancies.

Acknowledgments:We would like to thank biorender (www.biorender.com) for the open access platform to create Illustrations.

Author contributions:Both authors participated in manuscript writing,data search, collection, analysis, and approved the final version of the manuscript.

Conflicts of interest:The authors declare no conflicts of interest.

Financial support:None.

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Peer review:Externally peer reviewed.

Open access statement:This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix,tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

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